diff --git a/platform-operate-api/src/main/java/com/lyms/platform/operate/web/facade/ApplyOrderFacade.java b/platform-operate-api/src/main/java/com/lyms/platform/operate/web/facade/ApplyOrderFacade.java
index 2c143f8..0bc2951 100644
--- a/platform-operate-api/src/main/java/com/lyms/platform/operate/web/facade/ApplyOrderFacade.java
+++ b/platform-operate-api/src/main/java/com/lyms/platform/operate/web/facade/ApplyOrderFacade.java
@@ -389,6 +389,11 @@ public class ApplyOrderFacade {
     public BaseResponse addOneSieveApplyOrder(SieveApplyOrderAddRequest sieveApplyOrderAddRequest, Integer userId) {
         SieveApplyOrderModel sieveApplyOrderModel = sieveApplyOrderAddRequest.convertToDataModel();
 
+        boolean existTwo = exists(sieveApplyOrderModel);
+        if (existTwo) {
+            return new BaseResponse().setErrorcode(ErrorCodeConstants.PARAMETER_ERROR).setErrormsg("标本号重复");
+        }
+
         //修改
         if (StringUtils.isNotEmpty(sieveApplyOrderAddRequest.getId())) {
             sieveApplyOrderModel.setId(sieveApplyOrderAddRequest.getId());
@@ -534,6 +539,34 @@ public class ApplyOrderFacade {
     }
 
     /**
+     * 检查标本号是否已存在
+     *
+     * @param sieveApplyOrderModel
+     * @return true 是 false 否
+     */
+    private boolean exists(SieveApplyOrderModel sieveApplyOrderModel) {
+        String number = sieveApplyOrderModel.getNumber();
+        List<SieveApplyOrderModel> sieveApplyOrderModels = mongoTemplate.find(Query.query(Criteria.where("number").is(number).and("yn").is(YnEnums.YES.getId())), SieveApplyOrderModel.class);
+        if (CollectionUtils.isNotEmpty(sieveApplyOrderModels)) {
+            int size = sieveApplyOrderModels.size();
+            if (size > 1) {
+                return true;
+            }
+            SieveApplyOrderModel model = sieveApplyOrderModels.get(0);
+            String modelIdDB = model.getId();
+            String modelIdWeb = sieveApplyOrderModel.getId();
+            if (StringUtils.isNotEmpty(modelIdWeb)) {
+                if (!modelIdDB.equals(modelIdWeb)) {
+                    return true;
+                }
+            } else {
+                return true;
+            }
+        }
+        return false;
+    }
+
+    /**
      * 调用华大接口
      *
      * @param sieveApplyOrderModel